CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN DIFFERENT EGYPTIAN CHICKEN FLOCKS
WafaaA.AbdEl-Ghany, HananA.Ahmed andAliZ.Qandoos
*1 2 3 WafaaA.AbdEl-Ghany, HananA.Ahmed andAliZ.Qandoos
1 PoultryDiseasesDepartment,FacultyofVeterinaryMedicine,CairoUniversity,12211Giza,Egypt
2 CentralLaboratoryforEvaluationofVeterinaryBiologics,Cairo,Egypt;ResearcheratFacultyofVeterinaryMedicine,
3 CairoUniversity,Egypt
Page Number(s):
1693-1700
Published Online First:
December 01, 2018
Publication Date:
December 01, 2018
ABSTRACT
Inthepresentstudy,phenotypicandgenotypiccharacterizationof Pasteurella multocida(P. multocida)strainsfrom differentEgyptianlayersandbreederschickenflockswascarriedout.Organswerecollectedfrom55chickensflocks represented39layersand16broilersandlayersbreedersinEgyptduringtheperiod2016-2017.Sixbirdsweretakenfrom eachsuspectedflockwithatotalnumber330birdsthatrepresenting234and96freshlydeadlayersandbreederschickens, respectively.Suspectedorganscontaining P. multocidaweresubjectedforisolationandidentificationoftheorganism,as wellastestingofpathogenicityinmice.Moreover,genotypiccharacterizationof P. multocidaisolatewasdoneusing PolymeraseChainReaction(PCR).Capsularandsomatictypingwasdone.Thephenotypicandgenotypiccharacterization resultsrevealedthat6flockswerepositivefor P. multocidaoutof55ones(10.9%).Outof330deadchickens,36(10.9%) werepositivefor P. multocida.All P. multocidastrainswerepathogenicformice.Capsulartypingof P. multocidausing multiplexPCRshowedthatallstrainsweretypeA,whilesomatictypingrevealedpresenceoftypes1and3.Itcouldbe concludedthat P. multocidaiscirculatinginlayersandbreederchickenflocksinEgyptcreatingsevereeconomiclosses. multocida,PCRisused(Townsend et al.,1998,Sellyei et
Keywords:
Pasteurella,PCR,LayersandBreeders,Egypt.