The objective of this study was to investigate the effect of graded amount of calcium salts of palm fatty acids (Ca-PFA) supplementation on dry matter intake, milk yield, milk fat, and milk fatty acid profile in lactating buffalo. Twelve multiparous early-lactating buffaloes were arranged in a 4 × 4 Latin-square design with a period length of 21 day. The 4 treatments were basal diet with supplementation of 0, 200, 400, and 600 g of Ca-PFA per day. The 3.5% fat-corrected milk yield, milk fat content, and milk fat yield showed a quadratic function with a transient maximumat 400 g/d of supplemental level. The concentration and yield of C16:0 and C18:0 increased linearly, whereas concentration of C16:1 and C18:2 passed a transient maximum and afterward decreased upon intake of Ca-PFA. The C16:0 yield tended to increase linearly, whereas C16:1 yield showed a quadratic function with increasing Ca-PFA intake. Cumulatively, increasing the Ca-PFA supplementation decreased the content and yield of de novo milk FA by 21.7% and increased preformed milk FA by approximately 10.0%. In conclusion, under the current feeding scheme, the elevated Ca-PFA intake increased milk and milk fat yields and the responses were maximal at 400 g/dof Ca-PFA supplemental level.