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Volume 30, No. (1), 2020 (February)
COMPARISON OF ANALYTICAL SENSITIVITY AND SPECIFICITY OF REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) AND REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR) FOR DETECTION OF LOCAL PESTIVIRUS A
Hammad-ur-Rehman1, A. Ahmad1, M. Rabbani, M. Y. Tipu3­

1University Diagnostic Lab, University of Veterinary & Animal Sciences, Lahore, Pakistan
2Department of Microbiology, University of Veterinary & Animal Sciences, Lahore, Pakistan
3Department of Pathology, University of Veterinary & Animal Sciences, Lahore, Pakistan
Corresponding Author: hammad.rehman@uvas.edu.pk; iffivet@uvas.edu.pk
DOI: https://doi.org/10.36899/JAPS.2020.1.0006
Page Number(s): 50-57
Published Online First: January 02, 2020
Publication Date: January 02, 2020
ABSTRACT

The main aim of this study was to compare reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription polymerase chain reaction (RT-PCR) in order to determine their analytical sensitivity and specificity using local characterized Pestivirus A. In order to compare the sensitivity of RT-LAMP and RT-PCR, serial 10 fold dilutions containing 9.91 x 1010 to 9.91 x 10-2 copies of cDNA of Pestivirus A, were prepared and tested. RT-LAMP proved more sensitive and was able to detect 9.91x100 copies of cDNA compared to RT-PCR (9.91x101). Both RT-LAMP and RT-PCR were found equally specific as no cross reaction with bovine herpes virus was observed.  Present study showed that RT-LAMP assay is highly sensitive, specific, rapid and can be used as an alternative to conventional RT-PCR, for the detection of Pestivirus A.
Keywords: Pestivirus A, Sensitivity, Specificity, RT-LAMP, RT-PCR
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).
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