SCREENING AND ASSESSMENT OF LACCASE PRODUCING TRICHODERMA SPECIES ISOLATED FROM DIFFERENT ENVIRONMENTAL SAMPLES
S. Ahmed, H. A. Siddiqui
S. Ahmed*, H. A. Siddiqui
1 Department of Botany, University of the Punjab, Lahore-54590
2 Institute of Agricultural Sciences, University of the Punjab, Lahore-54590
Published Online First:
June 01, 2015
Publication Date:
June 01, 2015
ABSTRACT
The cultivation conditions and high fungal biomass do not support essentially for high laccase yields. In the present study, it was attempted to select Trichoderma species for the production of laccase in submerged fermentation. For this purpose, 88 different fungi were isolated, out of which 29 isolates belong to 7 species of Trichoderma. Laccases were usually produced in low concentrations by laccase producing fungi, but high concentrations were obtained with the addition of different supplements to media and optimized cultural conditions. 29 isolates belonging to 7 species of Trichoderma were screened as laccase producers, based on decolorization of guaiacol. Trichoderma harzianum, isolated from industrial wastewater was found to be best potential laccase producing fungus while Trichoderma viridae showed the least growth. The rest of the Trichoderma species showed variable behaviour for laccase activity. Commission (EC) which oxidize diphenols and allied
Keywords:
Trichoderma spp, Laccase, Guaiacol, Environment, Pakistan.