ffect of different oligosaccharides on growth, yield, protein, and oil content in soybean. Two field experiments were conducted in 2021 and 2022 at the Bangladesh Institute of Nuclear Agriculture (BINA) Mymensingh, Bangladesh. Three oligosaccharides namely Chitosan oligosaccharides (COS), Alginate oligosaccharides (AOS) and Oligogalacturonides (OGA) were applied with 50 ppm, 70 ppm and 100 ppm on Binasoybean-2, respectively. In both years, higher soybean yield was obtained from different concentrations of OGA, which increased nearly 7-12% in 2021 and 10-11% in 2022 over control. Moreover, 100 ppm OGA provided a seed yield was 2639 kg ha^-1, which was the most optimal concentration for yield than other treatments. Oligosaccharides are all able to enhance protein content, and 100 ppm AOS (40.77%) produces the highest protein content which is 16-17% higher than control at 2022. Furthermore, the oil content shows a similar trend to the protein content, and the highest oil content was still generated by 100 ppm AOS (19.78%). Among the fatty acids, maximum oleic acid was obtained from 50 ppm COS (3.43%) and linoleic acid from 100 ppm COS (28.67%). Taken together, the use of oligosaccharide might be a sustainable approach to maximize soybean yield and quality, OGA has been shown to significantly increase soybean yields, with a recommended application concentration of 100 ppm.