THE PARTIAL AMINO ACID SEQUENCE OF LEAF-NOSED VIPER (ERISTICOPHIS MACMAHONII) S NAKE HEMOGLOBIN.
H. Waheed, S. K. Doman, A. Ahmed
1, 2 3 3* H. Waheed, S. K. Doman, A. Ahmed
1 H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of
2 Karachi, Karachi-75270, Pakistan; Dow University of Health Sciences, Karachi-74200, Pakistan
3 Chapman University School of Pharmacy, Irvine, CA 92618, USA
Page Number(s):
1622-1628
Published Online First:
December 01, 2018
Publication Date:
December 01, 2018
ABSTRACT
The hemoglobin from Leaf-nosed Viper (Eristocophis macmahonii) was analyzed to better understand interspecies relationship among various snakes at the molecular level. After isolation, the hemoglobin from erythrocytes was analyzed by cation-exchange chromatography. The chains from hemoglobin were separated by reversed phase-high performance liquid chromatography. The N-terminal amino acid sequences of globin chains were obtained by the process of Edman degradation in an automated protein sequencer using an online Phenylthiohydantoin (PTH) analyzer. The obtained sequences were compared using online pairwise sequence alignment tool and Clustal Omega. The N- A I II terminal protein sequence results revealed that two hemoglobin components, comprised of ±, 2and 2 -globin chains A I are expressed in Leaf-nosed viper. The ±-globin chain showed highest similarity with Sindhi krait, 2with Texas indigo II snake and 2-with Indian cobra and interestingly with Blue-lipped sea krait (sea snake). N-terminal sequence alignment studies also showed some important amino acid substitutions that may affect iso-Hb composition and function. viper. Abbreviations: RP-HPLC, reversed phase-high performance liquid chromatography; IEX, ion-exchange; Em, Eristocophis macmahonii; TFA, trifluoroacetic acid; PTH, phenylthiohydantoin. physiological and ontogenetic factors (Lukin and Ho,
Keywords:
Eristocophis macmahonii; hemoglobin characterization; evolution; amino acid sequence; snakes; Leaf-nosed