CHANGESINTHEEXPRESSIONOFADIPONECTINANDITSRECEPTORS(A DIPOR1 ANDADIPOR2)INSEXUALLYIMMATUREANDSEXUALLYMATURESHEEPTESTES
ManBai, LiminSun, JiaZhao, GuishanZhang, WeiMa, LujieXiang, JiarongLi, ChaoJia, andHuaizhiJiang.
* ManBai, LiminSun, JiaZhao, GuishanZhang, WeiMa, LujieXiang, JiarongLi, ChaoJia, andHuaizhiJiang.
1 CollageofAnimalScienceandTechnology,JilinAgriculturalUniversity,Changchun130118,China.
Published Online First:
August 01, 2018
Publication Date:
August 01, 2018
ABSTRACT
Recentevidenceshaveindicatedthatadiponectinanditsreceptorshaveaneffectonmalereproduction.Theobjectiveof thisstudywastodetecttheexpressionofadiponectin,AdipoR1andAdipoR2insexuallyimmatureandsexuallymature sheeptestesusingqRT-PCRandWesternblot.Theresultsshowedthatadiponectin,AdipoR1andAdipoR2wereall expressed insheep testes. The expressionof AdipoR1 and AdipoR2 mRNAs(p<0.05 and p<0.01) as wellas the expressionofAdipoR1andAdipoR2proteins(p<0.01andp<0.05)insexuallymaturetestesweresignificantlyhigher thanthatinsexuallyimmaturetestes.However,therewasnosignificantdifferenceintheexpressionof adiponectin mRNA(p>0.05)insexuallyimmatureandsexuallymaturetestes,theexpressionofadiponectinproteininsexually maturetesteswassignificantlyhigherthanthatinsexuallyimmaturetestes(p<0.01).Itisthefirsttimetocomparethe mRNAandproteinexpressionofadiponectinanditsreceptorsinsexuallyimmatureandsexuallymaturesheeptestes. and AdipoR2wereexpressedinhumanandratpituitary
Keywords:
adiponectin,AdipoR1,AdipoR2,sheep,testes,qRT-PCR,Western-blot