PRODUCTION OF TRANS-RESVERATROL IN CALLUS TISSUE OF ÖKÜZGÖZÜ (VITIS VINIFERA L.) IN RESPONSE TO ULTRAVIOLET-C IRRADIATION

N. Keskin, B. Kunter

N. Keskin and B. Kunter

1 Yüzüncü Yıl University, Faculty of Agriculture, Department of Horticulture, 65080 Van, Turkey
2 Ankara University, Faculty of Agriculture, Department of Horticulture, 06110 Ankara, Turkey

Corresponding Author: nurhan_keskin@yahoo.com
Page Number(s): 197-200
Published Online First: June 01, 2010
Publication Date: June 01, 2010

ABSTRACT

In this study, the effects of ultraviolet (UV) irradiation time, incubation time and callus age were investigated for trans-resveratrol induction in callus cultures of Vitis vinifera L. cv. Öküzgözü. Callus tissues were exposed to 254 nm UV light at 10 cm distance from the source for 10 and 15 minutes by opening covers of the petri dishes in a sterile cabinet. High Pressure Liquid Chromatography was used for determining trans-resveratrol production and concentrations were recorded at 0, 24, 48 and 72 hours after beginning of incubation. The highest trans-resveratrol concentration (62.23 g/g callus fresh weight) was determined at 48 hours of 12 -day-old callus cultures irradiated for 15 min. 15 min UV irradiation period was found to be effective for induction of trans-resveratrol production and thus callus cultures could be convenient for trans-resveratrol production.

Keywords: Öküzgözü (Vitis vinifera L. cv.), trans-resveratrol, callus culture, UV irradiation
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).


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