1Department of Livestock and Poultry Production, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan; 2Department of Breeding and Genetics, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur- 63100, Pakistan; 3Buffalo Research Institute, Pattoki-55300, (Punjab), Pakistan; 4Department of Theriogenology, Faculty of Veterinary and Animal Sciences, Gomal University, Dera Ismail Khan-29220, Pakistan; 5Faculty of Veterinary Sciences, University of Poonch, Rawalakot