Uterus is an important reproductive organ for embryonic growth and development of viviparous animals. Its normal function is regulated by complex endocrine system and multiple genes. NRDR is a retinoic acid metabolizing enzyme with strong retinol oxidation and retinoaldehyde reduction activities. Its function in the reproductive system has been partially studied.In this study, the expression distribution and change of NRDR in in different physiological states and different developmental stages of mouse uterus will be clarified, so as to provide an experimental basis for further research on its function in uterus. Using immunohistochemistry and RT-qPCR, NRDR was proved mainly expressed in mouse endometrial byimmunohistochemistry. The expression of two different isoforms NRDR-1 and NRDR-2 in mouse uterus increased gradually with the development of the mouse, and peaked at 15 day after birth. The expression of NRDR-1 has no significant difference during estrous and diestrus in mouse uterus. However, the expression of NRDR-2 in estrous period was significantly higher than that in diestrus; NRDR two isoforms increased during embryo recognition and implantation in mouse uterus, and their expression decreased after implantation. The experimental results of the above expression patterns indicated that NRDR played a very important role in the mouse uterus, which was related to the estrus cycle and embryo implantation, among which the isoforms NRDR-2 might play a major role. The above results provide a basis for further study of the function of NRDR in uterus.The detection of the expression of NRDR in the uterus can provide experimental basis for understanding the molecular mechanism of development and other physiological processes in uterus.