S. Rahman, A. Ahmad and S. Bhatti
1 Department of Microbiology, University of Veterinary and Animal Sciences, Lahore.
A study was conducted to evaluate the growth promoting effect of FCS and its proper dilution in cell culture media for persistent cultivation and maintenance of BHK-21 cell culture, by comparing it with other animal sera. Different animal sera of different species as cattle calf serum , Allanto-amniotic fluid , Sheep serum , Poultry serum and Goat serum @ 10% were added to cell culture medium (M 199, Biomedicals; USA). A fast and reliable technique, MTT (3 {4, 5-Dimethyzol-2-Yl} 2, 5-Diphenyl Tetrazolium Bromide) Assay was employed to measure the cell densities in small amount of cell culture media by measuring OD values under 570nm, with 630nm as a reference read out. It was concluded that FCS is the best growth promoting agent for BHK-21 cell line and 5-7.5 % concentration has the desired effect of persistent cultivation and maintenance of BHK-21 cell line.
Cite Score: 1.3
JCR Year: 2025
Web of Science (SCIE)
SCOPUS (Q3)
Journal Impact Factor: 0.5
HEC Category: W
Print ISSN: 1018-7081
Electronic ISSN: 2309-8694
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