Isoflavone has an effect on countering the progression of skin aging, while probiotics boost the absorption of nutrients and maintain the skin tissue. Fermentation of isoflavone might be more beneficial for inducing cytokine system and boosting the skin regeneration. There is limited information about the difference of effect between the various formulation of fermented cow milk and soy milk towards the skin regeneration via epidermal growth factor (EGF) signaling. Twenty-five female rats were divided into 5 group for 12 weeks supplementation of fermented soy and cow milk by oral gavaging. Specific probiotics composition was utilized for fermenting formulation cow and soymilk consist (Lactobacillus bulgaricus, Streptococcus thermophiles, Lactobacillus acidophilus, and Bifidobacterium bifidum). Our data showed that there are different characteristics of stool and its macroscopic appearance after different probiotics composition of fermented milk treatment. Histology results showed that the epidermal skin thickness was found higher in every treatment group. Similar results are found from EGF protein levels. Fermented cow milk with Bifidobacterium bifidum and Lactobacillus acidophilus were found to have a thicker epidermal and higher level of EGF protein level. Milk fermented by Bifidobacterium bifidum showed the most significant effect in regenerating skin tissue. Therefore, it might prevent the skin aging process compared to the other formulations. Compared to conventional fermented milk, the composition of soy milk might enhance this positive effect of fermented milk on the skin.