The present study was planned on glass aquaria in triplicate groups to check the effect of limiting amino acid (lysine, threonine plus methionine) supplementation in low crude protein diets. Fish fry was served twice a day with five experimental diets designated as; Treatment 1 (35% CP with NRC recommended doses of amino acids) as control diet, Treatment 2 (34.36% CP with 5 % amino acids supplementation), Treatment 3 (34.36% CP with 10 % amino acid supplementation), Treatment 4 (33.60% CP with 10 % amino acids supplementation), and Treatment 5 (33.60% CP with 20 % amino acids supplementation).@ three percent of body weight. Growth results showed a significant increase in specific growth rate, weight gain and carcass protein (60.93±00.15a) in treatment served with T5. Moreover, the feed conversion ratio was improved in treatment administered with augmented levels of limiting amino acid supplementation. However, hematological assay of Labeo rohita fry showed non-significant results excluding thrombocytes (416.33±9.33a), which were increased significantly in treatment 4. Level of dispensable and indispensable amino acids including methionine (02.20±00.12ab), phenylalanine (03.33± 00.29a), lysine (04.34± 00.08a) and threonine (02.58±00.10a )were markedly increased in treatments served with an augmented level of amino acids.