A 3×3 factorial feeding experimental was performed to find out the role of citric acid and phytase supplementations and interaction in improving body chemical composition and bone mineralization by adding them in plant based diet of rohu (Labeo rohita) juveniles. At first, the basal diet having citric acid at three different levels was supplemented i.e. 0%, 1.5% and 3%, and then three different phytase levels (0 FTU kgˉ¹, 750 FTU kgˉ¹ and 1000 FTU kgˉ¹) were supplemented further with each level of citric acid. Hence, total 9 experimental diets were formulated in this feeding trial. The results revealed that the concentration of total ash, crude protein and dry matter content significantly increased (p≤0.05), when treated with citric acid and phytase. However, contents of crude fat showed a reduced level in the carcass of juveniles by feeding both additives. The supplementation of citric acid and phytase both significantly (p≤0.05) affected the bone mineralization of fish. An increasing trend was observed for the body proximate analysis and bone mineral concentrations with an increase of supplements level in the diet. Conclusively, the results of this experiment showed that the supplementation of citric acid and phytase in the diet of L. rohita improved body composition and bone mineralization.