Clitoria ternatea is one of the medicinal plants of fabaceae family that have been traditionally useful in herbal medicine. In present study, the invitro antimicrobial, antioxidant and enzyme inhibition assay of polar and non-polar extracts/fractions of Clitoria ternatea seeds were evaluated. The antimicrobial activity was done by well diffusion assay while MIC was done by serial dilution method. Crude ethanolic extract showed maximum antibacterial activity against B. subtilus (17.6 mm ±0.17). Ethyl acetate extract showed maximum MIC at 0.156 μg/mL against E. coli. In addition, Crude methanolic extract showed maximum antifungal activity against Aspergillus parasiticus (16 mm ±0.27). The antioxidant activity was done by DPPH and FRAP assay. Crude methanolic extract showed maximum antioxidant activity by DPPH assay (86.26%) while crude methanolic extract exhibit maximum reducing power 370.19 % ± 20.50. Moreover, n-hexane extract possessed highest phenolic content (272.16 ± 17.96 GAE µg/g), crude methanolic extract possessed highest flavonoid content (275.5 ± 8.24 GAE µg/g). The highest enzyme inhibition assay was observed in crude methanolic extract (α-amylase), n-hexane extract and water-soluble residue exhibited 50% pepsin enzyme inhibition. The results concluded that most of the extracts/fractions obtained from Clitoria seeds showed significant biological activities and can act as promising source of natural therapeutic agents and can be used for future drug developmental studies.