1Department of Livestock and Poultry Production, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan; 2Buffalo Research Institite, Pattoki, Distt. Kasur, Pakistan; 3Department of Breeding and Genetics, Faculty of Animal Production and Technology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur- 63100, Pakistan; 4Gomal College of Veterinary Sciences, Gomal University, Dera Ismail Khan, Pakistan