The aim of the study was to investigate the effects of feeding programs in combination with supplemental lipotropic
agents on performance, ascites-related indices, blood metabolites and breast meat quality in broiler chickens reared at
high altitude (2,200 m above sea). A total of 450 day-old Ross broiler chickens were randomly assigned to 6 treatments
with 5 pens of 15 birds per each. The experiment was carried out in a completely randomized design with 2 × 3 factorial
arrangement, including two feeding program (ad libitum or early feed restriction) and three status of lipotropic
supplementations (a control diet, 150 mg/kg carnitine as L-carnitine, and 1,000 mg/kg choline as choline chloride). The
results showed that dietary supplementation with carnitine improved body weight gain and feed conversion ratio (FCR)
in broilers (P≤ 0.05). The relative weight of the heart was lower in broilers fed choline supplemented diet compared with
control group (P≤ 0.05). Feeding broiler chickens with carnitine increased the serum concentration of glucose (P≤ 0.05).
However, supplementation of the diet with carnitine reduced serum concentration of triglycerides (P≤0.05).
Supplemental carnitine reduced the susceptibility of the meat (thiobarbituric acid number) to lipid peroxidation (P≤
0.05). The ascites-related indices, including the weight of right ventricle (RV) and the ratio of right-to-total ventricular
weight were lower in broilers which received carnitine and choline supplementation which reared under early feed
restriction (P≤ 0.05). In conclusion, supplemental carnitine improved growth performance, ascites-related indices and
meat quality of broilers. Besides, early feed restriction improved ascites-related indices in broiler chickens reared at high
altitude.