The aim of the present study was to investigate the dose-response effect of selected Australian EOs on in vitro rumen
fermentation characteristics in order to find a combination of EO and dose that may be effective against methane and
ammonia-N, while maintaining rumen fermentation. Selected EO treatments significantly (P<0.05) reduced methane
(between 25% and 97% reduction) and ammonia-N (between 19% to 70% reduction) concentrations when compared to
control, and change occurred in a dose-dependent manner. However, other fermentation parameters were also reduced in
all of these, but in Santalum spicatum EO treatment the effect on methane (50% reduction) and ammonia-N (59%reduction) were greater than the effect on overall fermentation (i.e. VFA reduced only up to 20%). Results of the present
study demonstrated that at doses tested, it was not possible to dissociate the effects of EOs on in vitro fermentation,
except in S. spicatum EO, where selected doses may reduce methane and ammonia N with less negative effect on overall
fermentation by rumen microbes.