Jatropha curcas is a tremendous source of biodiesel with seeds containing 30-40% oil contents. The plant also exhibits diverse medicinal values, but Jatropha production is negligible due to fluctuations in weather causing seed dormancy. The micropropagation of this economically important plant is the basic requirement of present era to cope its high demands for biodiesel production. Applications of various phytohormones significantly increase the micropropagation of Jatropha. The present study reveals that MS added with BAP (1.0 mg/l) media produced seeds germination and shoot induction in the shortest possible time (10.18 d) and (10.60 d), respectively. , While MS media added with IBA (1.0 mg/l) presented less days to root initiation (10.64). The maximum germination percentage (73.49%) was recorded on media containing BAP (1.0 mg/l), while less bacterial contamination (1.61%) was recorded in MS plain media. MS media added with GA3 (1.0 mg/l) exhibited the seeds germination in a very short time. The highest number of shoots (80) was found in MS supplemented with BAP (1.0 mg/l) in the seedlings. The MS media having 2, 4-D + BAP (0.5 mg/l) depicted maximum production of leaves-derived callus initiation (100%). Then the plantlets were subjected to acclimatization and subsequently transferred to soil that reached to maturity and produced normal seeds. The optimized protocols for micropropagation would be beneficial for the mass production of economically important Jatropha plant.