Potato cyst nematode (PCN), Globodera pallida and G. rostochiensis have been reported to cause severe decline potato yield and prolonged soil contamination. The presence of the parasites in potato rhizosphere made the question to control them very quickly. Therefore, the present study was conducted to control the G. rostochiensis using three different nematicides, ethoprophos (Mocap 10 G), fosthiazate (Nemathorin 10 G), and fluopyram (Velum Prime SC 400), and a biocontrol agent Paecilomyces lilacinus strain PL1 (Bio Nematon SL). Nematicide applications were carried out using label dosages and recommended application methods. The field experiments were conducted in two different naturally infested locations were the site one (Niğde) contained sandy and loam soil, and site (İzmir) contained clay soil conditions. Molecular confirmation was also done to confirm the G. rostochiensis species in both locations using PITSr3, PITSp4, and ITS5 primers in multiplex PCR. The results revealed that the initial population densities were found to be statistically similar among the plots, and significant reductions in cyst and egg +J2 population were recorded after harvesting. The highest reduction in Niğde was recorded by using the fluopyram (82%), followed by fosthiazate (74%), ethoprophos (65%), and P. lilacinus (58%). In İzmir, overall efficacy of the applied doses was recorded lower were, fluopyram recorded (73%), followed by fosthiazate (56%), ethoprophos (51%), and P. lilacinus (41%). The yield results corresponding to applied doses and nematode suppression was recorded as fluopyram increases the yield up to 35% followed by fosthiazate (33%), ethoprophos (30%), and P. lilacinus (26%) in Niğde. In İzmir the maximum yield increased was recorded for fluopyram (28%) followed by fosthiazate (26%), ethoprophos (23%), and P. lilacinus (22%), respectively. Thus, the results concluded that all applied nematicides are more effective in sandy soil than clay, and the nematicides fluopyram and fosthiazate were recorded as more effective and showed good potential in suppressing the G. rostochiensis population and enhancing the potato yield. Additionally, P. lilacinus offers a sustainable biological alternative. These results showed the importance of integrating chemical and biological strategies and considering soil characteristics for effective PCN management in potato production systems.