A study was carried out on Labeo rohita fingerlings to assess the effects of various plant oils as a substitute for fish oil (FO) for 70 days. A total of 270 fingerlings (15 fish/tank) were distributed in V-shaped tanks each with three replicates. Total six experimental diets were formed. Test diet-I (control) consisted of FO, whereas test diets-II, III, IV, and V comprised of sunflower, corn, canola and palm oils, respectively, and test diet-VI composed of mixture of all four plant oils. Results of this study showed that plant oil mixture based test diet-VI fed fingerlings showed maximum specific growth rate (1.92±0.001), weight gain (19.16±0.01 g) and lowest feed conversion ratio (1.32±0.02). While test diet-IV (canola oil) showed least growth performance. The fingerlings fed with test diet-VI had maximum digestibility i.e., crude fat (CF) (82.20±0.30%), crude protein (CP) (74.79±0.14%) and gross energy (GE) (73.51±0.21%). The lowest nutrient digestibility values for CP, CF, and GE were noticed in fingerlings fed with test diet-IV. In the case of body composition, test diet-VI showed maximum CP and ash values while having the least moisture and fat values. The outcomes of this research revealed that using plant oil mixture as a substitute for FO improved all growth parameters, carcass composition, and digestibility of L. rohita.