ICAM2 is a member of the immunoglobulin superfamily and plays an important role in inflammatory response and xenograft rejection. This study aimed to analyze the expression regulation pattern, sequence characteristics and potential biological functions of ICAM2 gene in the Banna mini-pig inbred lines. The results showed that the full-length CDS of ICAM2 gene was 834 bp, encoding 277 amino acids, including 11 exons and 10 introns. The ICAM2 gene contained two conserved domains, which were members of the ICAM_N family and the Ig superfamily; the gene has a transmembrane helix and a signal peptide sequence. Multi-species phylogenetic tree analysis revealed that the gene was highly conserved in Rodentia, Primates, Perissodactyla and Artiodactyla. Protein-protein interaction network analysis indicated that ICAM2 interacted closely with ICAM1, ITGAL, ICAM3, ITGB2 and MSN. ICAM2 mRNA was highly expressed in tissues such as the liver, spleen, lymph nodes and tonsils, and its protein was mainly expressed in the cytoplasm. In summary, these findings laid the foundation for future studies on the role of the ICMA2 gene in key biological processes such as the immune system of BMI pigs and xenotransplantation immune rejection.