Manuscript Abstract

EFFECT OF G.13700A>G AND G.25783C>T IN PIT-1 GENE ON GROWTH TRAITS OF DUROC, LANDRACE, YORKSHIRE PIG
Xinxing DONG, Ziyu LIU, Xiaoyi WANG, Qiang CHEN, Gaicui BAI, Guoxiang LAN, Qian WANG, Mingli LI, Dawei YAN, Shaoxiong LU

X. DONG¹*, Z. LIU², X. WANG³, Q. CHEN⁴, G. BAI⁵, G. LAN⁶, Q. WANG⁷, M. LI⁸, D. YAN⁹, S. LU¹⁰

¹ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
² Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
³ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
⁴ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
⁵ Dehong Animal Husbandry Station, Dehong 679399, Yunnan, China,
⁶ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
⁷ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
⁸ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
⁹ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,
¹⁰ Faculty of Animal Science and Technology, Yunnan Agricultural University, Fengyuan Road 452, Panlong, Kunming 650201, Yunnan, China,

Corresponding Author: 86127447@qq.com
Page Number(s): 1286-1291
Published Online First: August 22, 2023
Publication Date: December 15, 2023
ABSTRACT

Pituitary specific transcription factor-1 (Pit-1) gene is responsible for pituitary development and growth hormone expression, which is considered a pivotal candidate gene for growth in pig. This study aims to detect novel single nucleotide polymorphisms (SNPs) in porcine Pit-1 gene, investigate its effect on growth traits in the Duroc, Landrace and Yorkshire pigs. Two novel polymorphisms were detected, one located in the intron 2 (g. 13700 A>G) and another located in exon 4 (g.25783 C>T) which caused an amino acid change from threonine to methionine (Thr36Met). At g.13700 A>G site, the frequency of A allele was higher than G allele in Duroc, Landrace and Yorkshire. At the g.25783 C>T site, it was remarkable differences in allelic frequencies and genotypic frequencies among the three pig breeds. This study demonstrated the significant genotype effect of Pit-1 on body weights at 70 d, age when reached 30kg, 50kg, 100kg and average daily gain from 30kg to 100kg. According to our results, the novel Pit-1 polymorphisms may be useful in pig selection as molecular markers and required in future studies.

Keywords: Pit-1, pig , growth traits, polymorphisms
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).


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