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Volume 28, No. (2), 2018 (April)

DIVERSITY OF PHYTASE PRODUCING NON-TOXIGENIC FUNGI ISOLATED FROM SOIL

A. Ahmad, A. A. Anjum, M. Rabbani, K. Ashraf, M. Nawaz, S. Sana, A. Asif

A. Ahmad1*, A. A. Anjum1, M. Rabbani1, K. Ashraf2, M. Nawaz1, S. Sana1 and A. Asif3

1 Department of Microbiology,
2 Department of Parasitology, University of Veterinary and Animal Sciences, Lahore;
3 Gomal College of Veterinary Sciences, Gomal University, Dera Ismail Khan, Pakistan
Corresponding Author: aliahmedvet@yahoo.com
Page Number(s): 467-473
Published Online First: April 01, 2018
Publication Date: April 01, 2018

ABSTRACT

In present study diversity of phytase producing fungi isolated from soil of livestock farms in 10 percent villages of Lahore district of Punjab was explored. A total of 260 fungal isolates were obtained by soil dilution technique from soil samples (n=29) and qualitatively screened for phytase production on plates of phytase screening medium. Twenty eight (n=28) isolates were positive for phytase production. Out of 28 phytase producing fungi,13 isolates were toxigenic as detected by thin layer chromatography. Out of 15 non-toxigenic fungi, selected isolates (n=12) were identified as Aspergillus niger (n=7) and Aspergillus flavus (n=5) by morphological method using macroscopic and microscopic characters. The native non-toxigenic phytase producing fungi can be used for cost effective enzyme production on industrial level.
Keywords: Soil, livestock farms, phytase, thin layer chromatography, morphological method and cost effective
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).
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