Article
Volume 27, No. (1), 2017 (February)
B. TuncerDepartment of Horticulture, Faculty of Agriculture, Yuzuncu Yil University, Van, Turkey
In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (Raphanussativus L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4°C to microspores that had been isolated in Gamborg’s medium containing 13% (w/v)sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5°C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v)sucrose (1/2 NLN-13) at a density of 40.000 microspore mL-1and transferred for culturing under dark conditions at 25°C. Colonies of callus were observed in Petri dishes 4–5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkır) (6.63 calli/Petri dish)and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties.
Impact Score: 0.65; h Index:51
SJR: 0.20
Web of Science (SCIE): Q3
SCOPUS (Q3)
Journal Impact Factor (JIF): 0.6; JCR 2026 ; Five Year JIF: 0.7
HEC Category: W
Print ISSN: 1018-7081
Electronic ISSN: 2309-8694
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