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Volume 27, No. (6), 2017 (December)
RECOMBINANT LENTIVIRUS VECTOR TARGETING BOVINE LXRΑ MRNA AND ITS SILENCING EFFECTS ON BOVINE MUSCLE SATELLITE CELLS
Y. Liu, P. Lib, L. Zhao, Z. Zhao, Y. Li, M. Liu, X. Yang, L. Zan

Y. Liu1+, P. Lib+, L. Zhao1, Z. Zhao2, Y. Li2, M. Liu1, X. Yang and L. Zan2,*
1College of Food Engineering and Nutritional Science, Shaanxi Normal University, 710062, Xi’an, Shaanxi, P. R. China
2College of Animal Science and Technology, Northwest A & F University, 712100, Yangling Shaanxi, P. R. China
These authors contributed equally to this work
Corresponding Author: zanlinsen@163.com
Page Number(s): 1847-1851
Published Online First: December 01, 2017
Publication Date: December 01, 2017
ABSTRACT

The liver X receptor α (LXRα) is a member of the nuclear hormone receptor superfamily which could regulate the transcription of the genes involved in cholesterol transportation. In this study, Lentiviral vector containing LXRα shRNAs were constructed and transferred in bovine muscle satellite cells. The results indicated that LXRα down regulated PPARα, PPARγ, ABCA1, LPL, ApoE genes after 48 h post-pLenti-03 viruses' infection. The silencing effects of bovine LXRα gene on related were significant. This work identifies bovine LXRα role in cholesterol metabolism.
Keywords: bovine LXRα gene, lentivirus, vector construction, gene silencing
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).
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