Seed priming is a pre-sowing treatment used to reduce stress effects in crops. Chilling stress is one of the stresses that affect the most of the important cereal crops.  In order to investigate the role of nitric oxide in mitigating chilling induced physiological variations of wheat (Triticum aestivum L.) at early seedling development, two experiments were conducted during the year 2012 and 2013 and the data was statistically analyzed in MSTAT-C computer package. Seven wheat genotypes after seed priming in 0, 10-4 and 10-5 M sodium nitroprusside (as nitric oxide donor) were grown according to completely randomized design with three replicates in optimum growth conditions. Nitric Oxide donor priming at 10-4 M concentration was found to be more effective in reducing cold stress. The study of physiological attributes of the seedlings revealed that NARC-2011, Chakwal-50, Punjab-2011 and Faisalabad-2008 are comparatively more tolerant to chilling stress in association with NO priming.