This study was carried out to enhance the digestibility of nutrients and minerals through acidification of phytase treated canola meal based diet. The canola meal based diet (2% live wet fish weight) was supplemented with two levels of phytase (0 and 500 FTU/kg) and three levels of citric acid (0%, 1.5% and 3%). The optimum level of phytase and citric acid for releasing chelated nutrients and minerals of canola meal based diet were found to be 500 FTU/kg and 3% respectively for Labeo rohita. The higher digestibility of nutrients; dry matter, crude protein, crude fat and gross energy and minerals; Ca, Mg, Mn, Fe, Zn, Cu, K, Na and P were absent in groups fed on supplemented diets as compared to the control. These findings provided the strong evidence of significant enhancement of digestibility of nutrients and mineral through acidification of phytase treated diet with citric acid were found to be 500 FTU/kg and 3% citric acid.