S. S. Bibi1, A. Rehman2*, R. A. Minhas1 and S. Janjua2*
1Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad;
2Bioresource Research Centre (BRC), 34-Bazar Road, G-6/4 Islamabad
Five different protocols, viz. Andleeb et al. (2012), Shrivastava et al. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (Panthera pardus) preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be the most efficient protocol with 83% extraction rate and revealed 100% amplification of FCA026 and FCA043 loci. Keeping in views the extraction and amplification efficiency, Phenol chloroform triton method is recommended for molecular studies of formalin preserved skin samples of Panthera pardus.
Cite Score: 1.3
JCR Year: 2025
Web of Science (SCIE)
SCOPUS (Q3)
Journal Impact Factor: 0.5
HEC Category: W
Print ISSN: 1018-7081
Electronic ISSN: 2309-8694
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