BIOLOGICAL PATHOTYPING AND RAPD ANALYSIS OF ASCOCHYTA RABIEI, FROM VARIOUS CHICKPEA GROWING AREAS OF PAKISTAN

N. Sarwar, S. Ashfaq, K. P. Akhtar, F. F. Jamil

N. Sarwar, S. Ashfaq, K. P. Akhtar, F. F. Jamil
1 Nuclear Institute for Agriculture and Biology, P.O. Box No. 128, Jhang Road, Faisalabad, Pakistan.

Corresponding Author: nigsrw55@gmail.com
Page Number(s): 882-887
Published Online First: June 01, 2013
Publication Date: June 01, 2013

ABSTRACT

Blight disease caused by the fungus Ascochyta rabiei is considered to be the major constraint to chickpea production. Twenty-one isolates of A. rabiei were obtained from blighted samples collected from major chickpea growing areas in Pakistan during 2006-08. Biological pathotyping on a set of chickpea differentials indicated that four isolates were highly virulent; two were less virulent, while rest of the isolates was moderately virulent. RAPD assay was used to investigate the genetic diversity among the isolates. Twenty primers with random nucleotide sequences were tested. A total of 49 bands were polymorphic. Dendrogram constructed from RAPD data indicated that there was a high genetic variability among the isolates collected from different locations and were grouped into three main clusters.

Keywords: Ascochyta rabiei, blight, chickpea, DNA, Pathotypes, Genotyping
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).


Download Statistics
This Manuscript
Full Text
24
downloads
Indicators
Metrics

Impact Score: 0.65; h Index:51

SJR: 0.20

Indexing
Status

Web of Science (SCIE): Q3

SCOPUS (Q3)

Journal Metrics
Current

Journal Impact Factor (JIF): 0.6; JCR 2026 ; Five Year JIF: 0.7

HEC Category: W

ISSN Details
Verified

Print ISSN: 1018-7081

Electronic ISSN: 2309-8694

Search the Journal

Use the fields below to search for articles by Title, Author, or Keywords.

All Downloads
Full Text
127,184
downloads
Supplementary
534
downloads