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Volume 20, No. (4), 2010 (December)

PRODUCTION OF GOAT POX VIRUS VACCINE FROM A LIVE ATTENUATED GOAT POX VIRUS STRAIN

F. Abbas, F. A. Khan, A. Hussain, F. Ahmed, M. Ahmed, S. Ahmed, M. Z. Ahmed, M. A. Attique, A. Wadood, M. K. Taj.

F. Abbas, F. A. Khan, A. Hussain, F. Ahmed, M. Ahmed, S. Ahmed, M. Z. Ahmed, M. A. Attique, A. Wadood and M. K. Taj.

Center for Advanced Studies in Vaccinology & Biotechnology, University of Balochistan, Quetta, Pakistanistan
* Veterinary Research Institute, Quetta, Balochistan
Corresponding Author: ferhatcasvab@yahoo.com
Page Number(s): 315-317
Published Online First: August 01, 2010
Publication Date: August 01, 2010

ABSTRACT

Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days.   Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers.   Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation.  TCID50 calculated for the pool virus was 10 5.5/ ml.  Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration.  Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 104.5/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 3.5/ml)  showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.
Keywords: attenuated, goat pox virus, confluent, monolayer, virulent, challenge
Open Access: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).
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