EFFECT OF TAXIFOLIN ON DOXORUBICIN-INDUCED OXIDATIVE CARDIAC DAMAGE IN RATS: A BIOCHEMICAL AND HISTOPATHOLOGICAL EVALUATION
M. N. Aldemir1, A. V. Kara2, R. Mammadov3, G. N. Yazici4, B. Cicek5, B. Yavuzer3, R. Coskun6, A. Sakin7, M. Gulaboglu8 and H. Suleyman3*
1Department of Medical Oncology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey; 2Department of Nephrology, 3Department of Pharmacology, 4Department of Histology and Embryology, 5Department of Physiology, 6Department of Cardiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan, Turkey
7Department of Medical Oncology, Bahcelievler Medipol Hospital, Istanbul, Turkey
8Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey
*Corresponding Author’s e-mail: halis.suleyman@gmail.com
ABSTRACT
Doxorubicin is a widely used anthracycline-derived broad-spectrum antitumoral antibiotic drug. However, cardiotoxicity due to doxorubicin treatment has warranted dose reduction or complete discontinuation in certain cases. The role of oxidative stress in the pathogenesis of doxorubicin-induced cardiotoxicity has been previously demonstrated. Against this background, this study aimed to investigate the protective effect of the potent antioxidant flavone taxifolin against possible oxidative heart damage biochemically and histopathologically induced by doxorubicin. Albino Wistar male rats were divided into three groups: healthy controls (HG), a group given doxorubicin alone (DG), and a group given taxifolin + doxorubicin (TDG). Taxifolin was administered orally at a dose of 50 mg/kg via gavage. Doxorubicin was injected intraperitoneally at a dose of 5 mg/kg. This procedure was repeated for 7 days. The results of the biochemical experiment showed that taxifolin significantly inhibited doxorubicin-induced malondialdehyde increases and glutathione decreases in heart tissues. In addition, taxifolin significantly suppressed the increases in cardiac damage markers, such as serum troponin I, creatine kinase, and creatine kinase-MB, induced by doxorubicin. Taxifolin treatment has also been histopathologically shown to alleviate doxorubicin-induced heart tissue damage. Accordingly, the results of the present study suggest that taxifolin may be useful in the treatment of doxorubicin-induced oxidative heart damage.
Keywords: Antioxidant, cardiotoxicity, doxorubicin, flavonoid, taxifolin.
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