EVALUATION OF ANGIOGENIC AND WOUND HEALING ACTIVITY OF BERGENIA CILIATA RHIZOME: IN OVO AND IN VIVO STUDY USING HISTOLOGICAL AND ANALYTICAL TOOLS

H. Imran^1*, U. Saleem², F. Anwar³, Z. Khan¹, F. Saleem¹, B. Ahmad³ and A. B. Waqar¹

¹University Institute of Medical Laboratory Technology, Faculty of Allied Health Sciences, The University of Lahore, Pakistan

²Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

³Riphah Institute of Pharmaceutical Sciences, Riphah International University Lahore, Pakistan

^*Correspondence to: Email:15hassanimran1991@gmail.com,

ABSTRACT

Bergenia Ciliata(Haw.) Rhizome (B. ciliata R) is used traditionally as a wound-healing remedy in hilly areas of Pakistan. This study was planned to evaluate the angiogenic and wound healing potential of the plant rhizome by in ovo and in vivo methods. For this purpose n-hexane, 80% ethanol-aqueous and aqueous extracts of B. ciliata R were prepared and analyzed for their phytochemical as well as their physicochemical characteristics. The antioxidant potential of all three extracts was determined using DPPH scavenging and Ferric reducing antioxidant power assay (FRAP assay). Based on the antioxidant potential of the extracts 80 % ethanol-aqueous extract was selected for angiogenic and wound healing studies using a Chorioallantoic membrane (CAM) assay and excision wound model, respectively. It was found that the 4 mg/mL 80% ethanol-aqueous extract exhibited the maximum angiogenic activity among all treatments. The macro and microscopic results showed that the 20% ointment accelerated wound healing as compared to the standard therapy and control group. Based upon the results of the CAM assay and excision wound model it is concluded that B. ciliata R extract may possess significant wound healing potential and therefore justify its folklore use as a wound healer.