PHYTOCHEMICAL PROFILING OF BIOACTIVE METABOLITES IN METHANOLIC EXTRACT OF TWO WILD SOLANUM SPECIES AND EVALUATION OF THEIR ANTIOXIDANT ACTIVITY.
N. M. George1, E. Abdelhaliem1 and M. Abdel-Haleem1*
1“Department of Botany and Microbiology, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt
*Corresponding author’s email: firstname.lastname@example.org; email@example.com
Medicinal plants abound in conventional medicines with antioxidant potentials that may be of value for the treatment of diseases, but with little or no scientific basis for their use. So, the present study aims to conduct a detailed investigation of phytochemicals present in two wild Solanum plants and also their potential use as an effective antioxidant agent. The objective of this present work was to identify the phytochemical compounds by using Gas chromatography–mass spectrometry technique and its biological activity of Solanum plant leaf extracts of methanol solvent systems were predicted using PASS online software. The methanolic leaf extract of Solanum nigrum and Solanum lycopersicum was tested to determine its chemical composition. The result of the preliminary phytochemical investigation in methanolic extract of Solanum revealed the presence of several bioactive secondary metabolites. In order to better understand the common uses of this plant, the antioxidant activity of the extracts was also measured using three separate assays.: “radical 2,2- diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, Ferric-reducing antioxidant power (FRAP) and 2,2-azino-bis(3-etilbenzotiazolin)-6-sulfonic acid (ABTS) scavenging assay”. In different antioxidant assays, the methanolic extract showed different IC50 values slightly higher than the standard to confirm the importance of medicinal plant's bioactive metabolites.
Keywords: GC-MS analysis, PASS, S. nigrum, S. lycopersicum, phytochemical screening, antioxidant activity