EVALUATION OF WATERBORNE ZINC OXIDE NANOPARTICLES INDUCED TOXICITY IN BIGHEAD CARP, HYPOPHTHALMICHTHYS NOBILIS
S. Aziz1, S. Abdullah1, F. Latif2*, and H. Anwar3
1Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan.
2 Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan
3Department of Physics, University of Agriculture, Faisalabad, Pakistan.
Corresponding author’s email: fariha.ansari44@yahoo.com ; farihalatif@bzu.edu.pk
ABSTRACT
Zinc oxide nanoparticles (ZnO-NPs) have widespread applications in various industries and cosmetics that raise concerns about their hazards to the environment. Eco-toxicological data show that nanoscale zinc oxide has harmful impacts on both aquatic species and human health. The co-precipitation method was followed to synthesize ZnO-NPs and X-ray diffraction (XRD) technique and scanning electron microscope (SEM) were used for characterization. The acute toxicity at 96h was determined by using Bighead carp as a model fish after the exposure to 11 various concentrations (2-50mg/L) of ZnO-NPs. The mean 96-h LC50 was measured as 22.24mg/L. Bighead carp showed different behavioral changes that lead to death during acute toxicity tests. Oxidative stress in terms of lipid peroxidation (LPO) and superoxide dismutase (SOD) was determined in fish gills and liver after chronic exposure to ZnO-NPs for 90 days and sampling was done at 15, 30, 45, 60, 75 and 90-day intervals. Significant alterations in SOD and LPO levels were determined in tissues of the liver and gills as compared to the control group. We conclude that induced toxicity mechanism of ZnO-NPs suspension in freshwater environments may be due to elevated oxidative stress.
Keywords: Bighead carp, Zinc oxide nanoparticles; Toxicity; Oxidative stress, Lipid Peroxidation
https://doi.org/10.36899/JAPS.2022.5.0536
Published first online April 26, 2022
INTRODUCTION
Nanomaterials are widely used in different research fields due to novel physico-chemical characteristics as compared to bulk materials (Khan et al., 2019). Engineered nanoparticles (NPs) can be naturally present in the ecosystem and necessarily discharged during construction, use, and disposal operations, indicating that a basic understanding of their physical state and extent of toxicity is required (Handy et al., 2008). For domestic applications and industries, metal oxide nanoparticles are widely used (Aitken et al., 2006). Zinc oxide nanoparticles (ZnO-NPs) are used in glass, ointments, cosmetics, dyes, optical filters, and cement industries (Rekha et al., 2010). Therefore, they enter from point and non-point sources in the aquatic environment and are taken by fish through gills, digestive tract, skin, and transported to the internal organs and tissues (Wang et al., 2009; Xiong et al., 2011) and then to humans (Yu et al., 2011; Handy et al., 2008). Due to its smaller particle size, penetration into the cell membrane becomes easier that leads to instability in cell membrane permeability and oxidative stress (OXS) (Ma et al., 2013). Within the cell, OXS is one of the most important mechanisms of nanomaterials toxicity that leads to the accumulation of O-2 (Superoxide radical) by Fenton type reactions that finally starts programmed cell death and DNA damage (Nel et al., 2006). Due to the key role of fish in the food web of aquatic ecosystem, they are considered as an important biomarker for assessment of entire ecosystems (Baker et al., 2014). Various research works have been done on ecotoxicity of nanomaterials, but only minimal information is present about bioavailability and the hazards of NPs in fish (Klaine et al., 2008). Carps are considered as a most common food source due to consumer preferences in Pakistan. But now a day, water pollution is becoming a major concern in the wetlands of Pakistan that adversely affects the freshwater living organisms. In intensive monoculture systems, mostly carps are cultured to reduce hunger and fulfill the global demand that enhanced variety of toxicants in aquatic systems (Gupta et al., 2016). Recently, research on fish has gained more attention as a model to study the toxic impacts of NPs. The present study was designed to find out acute toxicity of nanoscale zinc oxide and its effects on behavior and oxidative stress in terms of lipid peroxidation (LPO) and superoxide dismutase (SOD) in the gills and liver of Bighead carp (Hypophthalmichthys nobilis) after exposure to sub-lethal dose (1/3rd of its respective 96-h LC50) for 90days.
MATERIALS AND METHODS
Preparation and characterization of ZnO-NPs: For the preparation of ZnO-NPs, sodium hydroxide and zinc sulfate were used. Distilled water was used for the formulation of solution. For the zinc sulfate solution, sodium hydroxide was added dropwise (in a molar ratio of 1:2) under continuous stirring for 12h. The filtered precipitates were collected and washed with deionized water. Then precipitates were dried at 100°C in an oven (Shel-Lab). Fine powder was prepared using agate mortar. After that collected powder was calcined in furnace (SNOL-LHM01) for two hours at 500°C. Sample in powder form was used to calculate average crystal size by X-ray diffraction (XRD) using the Scherrer formula d = 0.89λ/B cos θ, where d represents the grain size; λ= wavelength of X-rays; B= full width at half maximum; θ = diffraction angle. Surface morphology of particles was checked by scanning electron microscope (SEM) (JEOL-JSM 5910).
Collection and Maintenance of Test Organisms: This experiment was done in the wet laboratory of Fisheries Research Farms of the University of Agriculture, Faisalabad, Pakistan. Freshwater fish, Bighead carp (90-day old) were placed in cement tanks after acclimation of two weeks and fed on commercial pellet fish feed (30% digestible protein and 3Kcal/g digestible energy)..
Acute toxicity: Fresh water fish, Bighead carp was selected for mean lethal toxicity (LC50) and lethality tests. Different concentrations of zinc oxide nanoparticles viz. 0, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 mg/L were tested. Ten fish were exposed to all above-described concentrations for 96-h, under constant conditions. After 24 hours, test solution was changed to fix concentrations in tanks. Sonication was done for about 60 minutes immediately prior to use to avoid particle aggregation. In the control group, no test solution was added. During the trial, the fish were not fed to reduce the absorption of the ZnO-NPs in feed and feces. During the experiment, water quality parameters like pH, temperature, total hardness, dissolved oxygen and natural 12:12 day /night photoperiod were retained (Table 1). Besides this, behavior of Bighead carp was observed in exposed and control group. Their movement, hyperactivity, equilibrium, air gulping activity and swimming pattern were checked (Almeida et al., 2010).
Table 1: Physico-chemical parameters used for the experiments.
Parameters
|
Unit
|
Mean
|
Analysis Method
|
Total Hardness
|
mg/L
|
150±0.38
|
Titration method
|
pH
|
_
|
7.5±0.04
|
pH meter
|
Temperature
|
°C
|
29±0.09
|
Temperature meter
|
Dissolved Oxygen
|
mg/L
|
6.2±0.14
|
Oxygen meter
|
Ammonia
|
mg/L
|
0.43±0.22
|
Titrimetric method
|
Electrical Conductivity
|
µSiemens/cm
|
525.32±0.10
|
Conductivity meter
|
Oxidative stress biomarkers: Freshwater fish, Bighead carp was exposed to sub-lethal dose (1/3rd of 96-h LC50) for 90 days. After every 15 days, sampling was done and oxidative stress in terms of lipid peroxidation level (LPO) and superoxide dismutase (SOD) was assessed in the gills and liver of fish. Fish organs were homogenized separately, using chilled PBS (phosphate buffer saline) in 1/4 ratio (weight/volume) by homogenizer. After that homogenate was centrifuged at 10,000 rpm, 4°C for 15 min. For analysis of LPO and SOD, supernatant was used.
I. Superoxide dismutase activity (SOD): The activity of SOD was observed by its potential to suppress the process of photoreduction of Nitroblue tetrazole (NBT) at 560nm (Giannopolitis and Ries, 1977). Activity was determined in gills and liver tissues of Bighead carp.
II. Lipid peroxidation: The level of lipid peroxidation was checked by calculating thiobarbituric acid reactive substance (TBARS) in the gills and liver tissues (Gatta et al., 2000).
Water quality management: During experiments, the physical and chemical parameters viz. dissolved oxygen, pH, electrical conductivity and temperature were calculated by digital meters viz. HANNA-9146, HANNA-99301, HANNA-8424, respectively. Proper oxygen was maintained in the glass aquarium by an automatic air pump. For the maintenance of the level of pH, NaOH (to decrease level of pH) and HCL (to increase level of pH) was used. In order to maintain total hardness of test the media ethylenediaminetetraacetic acid (EDTA) and salts of magnesium sulfate (MgSO4) and calcium sulfate (CaSO4) were used. Sodium (Na), Magnesium (Mg), Carbon dioxide (CO2), potassium (K), calcium (Ca), and total ammonia and total hardness of test environment were recorded by following method of A.P.H.A. (2005).
Statistical analyses: To determine mean lethal concentrations of ZnO-NPs on Bighead carp, probit analysis method was done with 95% confidence interval (Hamilton et al., 1977) and all experiments were performed in triplicate. SPSS (Statistical Package for Social Sciences) was used to analyze statistically. Statistical differences and similarities within all variables were determined by Analysis of Variance. To compare means, Tukey’s/Student Newnan-Keul test was used.
RESULTS
Characterization of ZnO-NPs: To measure the average crystal size of samples, XRD was used. The X-ray diffraction pattern of ZnO-NPs is shown in Fig. 1. At 2θ, diffraction peaks 31.73°, 34.44°, 35.28°, 47.57°, 56.64°, 62.83°, 66.42°, 67.98° referred to as Planes of XRD as (100), (002), (101), (102), (110), (103), (112) and (201), respectively (Table 2). The average measured size was 53nm. SEM is important for sample scanning and knowledge about the shape of particles. Fig. 2 represents that zinc oxide manufactured NPs have a clear wurtzite-hexagonal crystalline arrangement.
Fig. 1: X-ray diffraction (XRD) Pattern of ZnO-NPs. Peaks showed average size of the particles
Fig. 2: Scanning electron microscopy (SEM) result of ZnO nanoparticles.
Table 2: Analysis of Peaks of X-ray diffraction Pattern of ZnO NPs.
No.
|
Pos. [°2Th.](deg.)
|
Theta (deg.)
|
d-spacing [Å]
|
FWHM [°2Th.]
|
Rel. Int. [%]
|
Hkl
|
1
|
31.7364
|
15.91473
|
2.82179
|
0.0974
|
59.45
|
100
|
2
|
34.2533
|
17.3412
|
2.62262
|
0.1248
|
45.0
|
002
|
3
|
35.2824
|
18.2346
|
2.23534
|
0.2166
|
100
|
101
|
4
|
48.589
|
23.7987
|
1.81945
|
0.2263
|
22.00
|
102
|
5
|
56.6559
|
28.32375
|
1.72794
|
0.0899
|
33.05
|
110
|
6
|
62.8342
|
31.45735
|
1.3767
|
0.1956
|
27.10
|
103
|
7
|
66.4243
|
33.2112
|
1.40286
|
0.2356
|
5.12
|
112
|
8
|
67.98623
|
33.8856
|
1.37954
|
0.1943
|
19.24
|
201
|
Acute toxicity of waterborne ZnO-NPs: Acute toxicity tests were conducted to calculate 96-hr LC50 and lethal concentration of ZnO-NPs for Bighead carp. Figure 3 shows fish mortality percentage against various concentrations.The mean 96-h LC50 and lethal concentration of zinc oxide nanopowder, for Bighead carp were calculated as 22.24 and 46.89 mg/L, respectively, at 95% confidence interval by using Probit analysis method (Table 3). Changes in behavior were observed after exposure to LC50 from 24-96 h. The behavioral changes induced in Bighead carp after ZnO-NPs exposure are present in Table 4.
Fig.3: Probability plot for success at 95% CI
Table 3: 96h acute toxicity of ZnO-NPs (mg/L) for Bighead carp
Fish specie
|
MeO-NPs
|
LC50
|
95%CI
|
LC99
|
95%CI
|
Pearson goodness of fit tests
|
|
|
|
(LCL-UCL)
|
|
(LCL-UPL)
|
Chi-Square
|
DF
|
P-value
|
Bighead carp
|
ZnO-NPs
|
22.24
|
17.95-26.23
|
46.89
|
40.75-57.88
|
2.65364
|
9
|
0.976
|
CI, confidence interval (mg/L); LCL, lower confidence limit (mg/L); UCL, upper confidence interval (mg/L);
Lethal Conc., lethal concentrations (mg/L); DF, degree of freedom
Table 4: Behavioral response of Bighead carp exposed to LC50 ZnO-NPs.
Behavior
|
Treatment groups
|
|
Control
|
Treated
|
Hyper activeness
|
-
|
+
|
Jumping
|
-
|
+
|
Air gulping
|
-
|
+
|
Equilibrium loss
|
-
|
+
|
Erratic swimming
|
-
|
+
|
Vertical position
|
-
|
+
|
- Absent, + Present.
Oxidative stress Biomarkers
Superoxide dismutase (SOD) activity: Chronic exposure of Bighead carp to sublethal dose (1/3rd 96-h LC50) of ZnO-NPs induced significantly variable superoxide dismutase activity in both liver and gill tissues. Liver tissues showed significant increase after 15 and 30 days followed by a sharp decrease at 45, 60, 75 and 90 days as compared to the control group in SOD activity, which increases the level of reactive oxygen species (Fig. 4A). Gills showed increased SOD activity after 15, 30, 45 and 60 days followed by significantly decreased rates at 75 and 90 days (Fig. 4B). In our present study, levels of superoxide dismutase is in the following trend: liver > gills.
Fig.4: Effect of sub-lethal concentration of ZnO-NPs on superoxide dismutase (SOD) activity (UmL-1) in Liver (A) and Gills (B) of Bighead carp.
Values represent Mean±SE (n=3). T1=control group, T2=ZnO-NPs exposed group
Lipid peroxidation (LPO): Thiobarbituric acid reactive substances (TBARS) are synthesized as a result of lipid peroxidation, which can be easily noticed by the TBARS assay. So, this assay was used in the present research as an effective symbol to detect lipid peroxidation. Bighead carp exposed to chronic exposure to sublethal dose (1/3rd LC50) of ZnO-NPs showed significant variation in TBARS level in liver and gills. Both organs showed significant increase in TBARS levels as duration increased than the control group (Fig. 5 A, B).
Fig. 5- Effect of ZnO-NPs on Thiobarbituric acid reactive substances (TBARS) level (mg/g protein) in Liver (A) and Gills (B) of Bighead carp.
Values represent Mean±SE (n=3). T1=control group, T2=ZnO-NPs exposed group.
DISCUSSION
Nanoparticles discharging into aquatic ecosystems may result in harmful effects on non-target animals such as fish and other aquatic organisms. The different sub-lethal concentrations of heavy metals can start accumulation of metals in target organs of fish that are settled at the last of the aquatic food chain and can disturb the food chain condition. The acute toxicity of ZnO-NPs was observed in common carp for 24 hrs and experimental fish showed the 50 % mortality rate at 30ppm concentration (Al-Taee et al., 2013). Subashkumar and Selvanayagam (2014) and Aziz et al. (2020) determined 4.897 and 31.15 mg/L LC50 of ZnO nanoparticle on selected carps. During present study, ZnO-NPs caused increased toxicity in Bighead carp as the particle concentrations increased. The toxicity of different types of NPs on different organisms depends upon various components viz. shape, morphology (triangular, spherical, and wire-shaped), chemical composition, size and structural properties. These mentioned factors significantly affect the physical action between target tissues and NPs (Albanese et al., 2012; Raza et al., 2016). Relatively few data are accessible in the literature that provides zinc oxide nanoparticles toxicity in fish (Kahru and Dubourguier, 2010). Toxicity is directly associated with the physico-chemical characteristics of ZnO nanoparticles. Water quality and trial species, both factors affect the acute toxicity of toxicants. Fish that are very sensitive to one metal at high or low levels may be less or even not sensitive to another metal in the environment (Hedayat and Safahieh, 2012). Thus, the outcomes of previous and our studies show that both exposure period and lethal concentrations may differ for different species. Bighead carp showed changes in hyperactivity, jumping, increased air gulping, equilibrium loss, rapid swimming and vertical position after 24-48 h of exposure. After 48h, Bighead carp became sluggish. At the end fish become inactive with taking vertical positions for sometimes before dying. Behavioral change in this study may be due to interaction of heavy metals with neurotransmitters acetylcholine. And fast and abrupt swimming may be due to muscle spasms that might be due to respiratory dysfunction and suffocation. Oxidative stress is an accessible factor to determine toxicity, because cells react to stress by raising a number of defensive reactions that can be easily calculated as changed enzymatic or genetic expression (Kovochich et al., 2007).In previous studies, it was noted that SOD activity affected after the nanomaterials exposure more than the activity of other enzymes (Hao et al., 2009; Aziz et al., 2021). This is in accordance with Hao and Chen (2012) who described a time-dependent significant reduction in SOD activity after ZnO-NPs exposure in common carp. The reduction in this antioxidant enzyme is expected to be caused by inducing oxidative stress of NPs. The same observations were found in the brain tissues of Tilapia zilli and O. niloticus exposed to 2 and 4 mg/L of silver NPs (Afifi et al., 2016). TBARS is one of the important factors used to find out LPO levels evoked by the biological processes in the organism (Ates et al., 2015). In this study, the TBARS assay showed a sharp increase in liver and gills LPO levels in Bighead carp exposed to sub-lethal dose of ZnO-NP (p < 0.05). This is in accordance with Zhao et al. (2013) who reported that the lipid peroxidation level was increased significantly at different concentrations of ZnO-NPs relative to control group, Increased liver TBARS level was observed on the 7th and 14th days in tilapia exposed to 10 mg/L ZnO-NPs suspension (p<0.05) in comparison to the control group (Kaya et al., 2015). In the same line of our data, some authors also determined high lipid peroxidation level in fish organs (Benavides et al., 2016, Yuan et al., 2015).
Conclusion: Bighead carp was used as a model organism to check 96-h acute toxicity of ZnO nanoparticles. Sub-lethal dose of zinc oxide nanoparticle has induced oxidative stress by causing alterations in both SOD and TBARs level due to excess of ROS (reactive oxygen species). The results develop an interest towards the security of aquatic biota and human beings.
Statement of conflict of interest: Authors have declared no conflict of interest.
Authors Contribution: SA executed the research. SA supervisor and planned the research. FL helped in compiling data and statistical analysis. HA helped in synthesis of nanoparticles.
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