DAILY USE OF CAMELLIA SINENSIS EXTRACT CAN PROTECT ACRYLAMIDE INDUCED ORGAN PATHOLOGIES IN MICE
C. Ara1*, Z. Zainab1, M. Khan1, Memoona1, A. Iqbal2 and K. Shahzad3
1Department of Zoology, University of the Punjab, Quaid-i-Azam Campus, Lahore- 54590, Pakistan.
2Department of Zoology, University of Veterinary and Animal Sciences, Lahore, Ravi Campus, Pattoki
3Department of Zoology, University of Okara, Okara.
*Corresponding Author’s E-mail: dr.chamanara@yahoo.com
ABSTRACT
Human beings are vulnerable to a variety of toxins. Nowadays concern has aroused to opt defensive mechanisms in the form of foodborne phytochemicals against these toxicants. Current research investigated the protective potential of green tea aqueous extract against Acrylamide (AA) induced organ toxicity. Eighty albino mice weighing (26±2g) divided equally into eight groups following CRD experimental mode. Three groups were orally exposed to various acrylamide concentrations (20mg/g, 40mg/g, 80mg/g B.W of mice), other three were treated with respective doses of AA along with green tea extract for thirty days regularly. Control (without treatment) and GE (only green tea extract) groups were also maintained during current experiment. Blood and organs (liver, kidneys and testes) were extracted on euthanasia of the animals after 24hrs of last treatment, for serological and histopathological studies, respectively. Aqueous extract of Camellia sinensis was also assessed for total phenolic contents and antioxidant activity. The observations indicated that AA intoxication reduced testes weight, size, testosterone and ALP level while increased kidneys and liver size as well as ALT, bilirubin, creatinine and urea levels significantly (p≤0.05) as compared to control group. Histopathological anomalies in testicular, hepatic and renal tissues were also obvious in AA group. However, pathologies were much suppressed when green tea is co-administrated with acrylamide except for the highest concentration of acrylamide. It is deduced by above mentioned results that AA exposure is noxious in mice and green tea extract showed protective potential against its toxicity.
Keywords: Acrylamide, Toxicity, Green Tea, Biochemistry, Histopathology. |
