ACIDIFICATION OF PHYTASE TREATED DIET IMPROVES THE BODY COMPOSITION AND BONE MINERALIZATION OF JUVENILE ROHU (Labeo rohita)
S. Z. H. Shah1*, M. Fatima2, M. Afzal3, S. M. Hussain4, H. Azmat2, M. Bilal5 and T. Ahmed6
1Department of Zoology, University of Gujrat, Gujrat, Pakistan; 2 Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan; 3Fish Nutrition Laboratory, Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad; 4Fish Nutrition Laboratory, Department of Zoology, Government College University, Faisalabad, Pakistan; 5School of life Science
and Food Engineering, Huaiyin Institute of Technology, Huaian, China; 6Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan.
* Corresponding author’s E-mail: email@example.com; firstname.lastname@example.org
A 3×3 factorial feeding experimental was performed to find out the role of citric acid and phytase supplementations and interaction in improving body chemical composition and bone mineralization by adding them in plant based diet of rohu (Labeo rohita) juveniles. At first, the basal diet having citric acid at three different levels was supplemented i.e. 0%, 1.5% and 3%, and then three different phytase levels (0 FTU kgˉ¹, 750 FTU kgˉ¹ and 1000 FTU kgˉ¹) were supplemented further with each level of citric acid. Hence, total 9 experimental diets were formulated in this feeding trial. The results revealed that the concentration of total ash, crude protein and dry matter content significantly increased (p≤0.05), when treated with citric acid and phytase. However, contents of crude fat showed a reduced level in the carcass of juveniles by feeding both additives. The supplementation of citric acid and phytase both significantly (p≤0.05) affected the bone mineralization of fish. An increasing trend was observed for the body proximate analysis and bone mineral concentrations with an increase of supplements level in the diet. Conclusively, the results of this experiment showed that the supplementation of citric acid and phytase in the diet of L. rohita improved body composition and bone mineralization.
Key words: Phytase, citric acid, rohu (Labeo rohita), bone mineralization, body mineralization, body proximate composition
Abbreviations: Phytase (PHY); Citric Acid (CA); Labeo rohita (L. rohita); Analysis of variance (ANOVA)