POST-EXPOSURE HISTOPATHOLOGICAL STUDIES OF ALBINO MICE LIVER TO SILVER NANOPARTICLES PREPARED FROM (Ocimum tenuiflorum) GREEN SYNTHESIS METHOD
A. Yaqub1, K. M. Anjum2, S. A. Ditta, F. Tanvir, and N. Malkani
2Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
1Department of Zoology, Government College University, Lahore, Pakistan
Corresponding Author’s email: atif@gcu.edu.pk; raviankhalid@gmail.com
ABSTRACT
Industrial and biomedical uses of nanoparticles have significantly increased in recent years. The Preparation of nanomaterials by conventional methods is posing serious hazards to the environment and the living beings in a variety of ways. Due to this setback, the development of some safer nanoparticles is deemed necessary for their potential applications instead of discontinuing their development and use altogether. During the present study, silver nanoparticles (AgNPs) using green synthesis approach were developed and characterization of the newly-synthesized AgNPs was performed by using ultraviolet (UV) visible spectroscopy, Scanning Electron Microscopy (SEM) analysis and Fourier-transform Infrared Spectroscopy (FTIR), revealing UV visible peak at 420 nm and confirming the formation of spherical-shaped AgNPs; SEM imaging also confirmed the spherical-shaped AgNPs with size ranging between 32 and 50nm; FTIR analysis designated the role of alkane and aromatic compounds from the plant extract as capping and reducing agent. Histopathology of albino mice liver was performed after dosing the mice with specific concentrations of the nanoparticles for 7, 14 and 21 days. Micrographs of the liver histology revealed cell distortion, necrosis, apoptosis, and detachment of hepatocytes and other signs of fibrosis in the mice liver exposed to the nanoparticles.
Keywords: Acute toxicity, Albino mice, Green synthesis, Histopathology, Oxidative stress, Silver nanoparticles.
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