CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN DIFFERENT EGYPTIAN CHICKEN FLOCKS
Wafaa A. Abd El-Ghany*1, Hanan A. Ahmed2 and Ali Z. Qandoos3
1Poultry Diseases Department, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt
2Central Laboratory for Evaluation of Veterinary Biologics, Cairo, Egypt; 3Researcher at Faculty of Veterinary Medicine, Cairo University, Egypt
*Corresponding author e-mail: firstname.lastname@example.org
In the present study, phenotypic and genotypic characterization of Pasteurella multocida (P. multocida) strains from different Egyptian layers and breeders chicken flocks was carried out. Organs were collected from 55 chicken’s flocks represented 39 layers and 16 broilers and layers breeders in Egypt during the period 2016-2017. Six birds were taken from each suspected flock with a total number 330 birds that representing 234 and 96 freshly dead layers and breeders chickens, respectively. Suspected organs containing P. multocida were subjected for isolation and identification of the organism, as well as testing of pathogenicity in mice. Moreover, genotypic characterization of P. multocida isolate was done using Polymerase Chain Reaction (PCR). Capsular and somatic typing was done. The phenotypic and genotypic characterization results revealed that 6 flocks were positive for P. multocida out of 55 ones (10.9%). Out of 330 dead chickens, 36 (10.9%) were positive for P. multocida. All P. multocida strains were pathogenic for mice. Capsular typing of P. multocida using multiplex PCR showed that all strains were type A, while somatic typing revealed presence of types 1 and 3. It could be concluded that P. multocida is circulating in layers and breeder chicken flocks in Egypt creating severe economic losses.
Keywords: Pasteurella, PCR, Layers and Breeders, Egypt.