STERILITY OF MALE AEDES AEGYPTI POST γ-RAY STERILIZATION
B. Ernawan1,2*, H.I. Sasmita1 and A. A. Parikesit3
1Center for Isotopes and Radiation Application-National Nuclear Energy Agency (CIRA-Batan). Jl. Lebak Bulus Raya No. 49 Jakarta 12440, Indonesia; 2Graduate Program of Department of Chemistry, Faculty of Mathematics and Science-Universitas Indonesia. Kampus UI Depok, West Java 16424, Indonesia; 3Bioinformatics Research Group, Department of Chemistry, Faculty of Mathematics and Science-Universitas Indonesia. Kampus UI Depok, West Java 16424, Indonesia.
*Corresponding Author’s email: beni_e@batan.go.id
ABSTRACT
Aedes aegypti (Ae. aegypti), a vector of dengue hemorrhagic fever (DHF), population control was developed using the sterile insect technique (SIT). Sterilization process was conducted using γ-ray sterilization. To support the SIT application, the sterility of male Ae. aegypti on the 1st-5th days post sterilization were studied. The first step of this research was the sterilization process of male Ae. aegypti with 70 Gy of γ-ray sterilization. Then, the sterile male Ae. aegypti mosquitoes on the 1st; 2nd; 3rd; 4th and 5th days after the sterilization process respectively were competed with un-irradiated males to mate with un-irradiated females. The number of eggs that were produced was incubated to determine the fertility of sterile male Ae. aegypti. The result showed that the average number of eggs produced from mating combination of sterile male Ae. aegypti mosquitoes on the 1st-5th days post sterilization, un-irradiated females and un-irradiated males was 1,135; 1,118; 1,243, 1,372 and 1,326. The average percentage number of eggs that did not hatch into larvae was 97.69%; 97.87%; 90.92%, 96.20%, and 86.91%. By analysis of variance (ANOVA), the number and percentage of the unhatched eggs were not significantly different at the level confident of 95%. The research showed that the mating ability and sterility of sterile male Ae. aegypti on the 1st-5th days post γ-ray sterilization show no significant difference.
Keywords: Sterile insect technique (SIT), sterility, Aedes aegypti, γ-ray sterilization.
|
