BIOLOGICAL EVALUATION OF LOCALLY PRODUCED RECOMBINANT PHYTASE IN BROILER CHICKS
F. Sabir1, M. Tayyab1*, A. R. Awan1, B. Muneer2, A. S. Hashmi1, M. Wasim1 and S. Firyal1
1Institute of Biochemistry & Biotechnology, University of Veterinary & Animal Sciences, Abdul Qadir Jillani (Outfall) Road, Lahore, Punjab, Pakistan; 2 Institute of Industrial Biotechnology, Government College University, Lahore, Punjab, Pakistan
*Correspondence Author’s email: muhammad.tayyab@uvas.edu.pk
ABSTRACT
Current study deals with the production of locally characterized recombinant thermostable phytase from Thermotoga naphthophila (PHYTN) and its biological evaluation in poultry broiler chicks. The PHYTN was produced from 16-liter LB medium using BL21 CodonPlus cells having pET-21a containing phytase gene from Thermotoga naphthophila. A total of 135 birds were divided into 5 groups each having 27 birds. Group A served as negative control, group B, C and D were given feed supplemented with 500, 1000 and 1500 IU/kg of locally produced recombinant phytase while group E served as positive control. The results depicted that locally produced recombinant phytase showed significant effect on bird’s weight gain, feed intake and feed efficiency ratio. Presence of 1000 IU/kg of phytase resulted in the weight gain in 3rd, 4th and 5th week of trial from 504.77±1 to 533.53±1.4, 767.9±2 to 823.73±2.84 and 999.8±7.66 to 1122.8±2g respectively when compared with the control. The group B, C and D showed an average feed intake of 2710, 2780 and 2790 g as compared to 2669 g for group A. The supplementation of 1000 IU/kg of locally produced phytase in feed resulted in the improvement of feed conversion ratio from 2.6 to 2.4. The study demonstrated that PHYTN is suitable for poultry feed industry and its domestic production will contribute in the economic availability of locally produced phytase for the poultry feed industry.
Key Words: PHYTN, Poultry Trial, Recombinant Thermostable Phytase, Thermotoga naphthophila, Phytate
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