COMPARISON AMONG DIFFERENT COTTON (GOSSYPIUM HIRSUTUM L.) GENOTYPES WITH RESPECT TO MORPHOLOGICAL, FIBRE QUALITY ATTRIBUTES AND EXPRESSION ANALYSIS OF CRY1AC GENE
M. A. Zia1, 2*, Z. K. Shinwari2, S. Ali1, S. H. Shah3, M. Anwar1 and G. M. Ali1
1National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC) Islamabad, Pakistan’ 2Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan
3Department of Agricultural Sciences, Allama Iqbal Open University, Islamabad, Pakistan
*Corresponding author email: amirzia_narc@yahoo.com
ABSTRACT
Cotton is a cash crop of Pakistan and it plays a key role in the economy of the country for earning of foreign exchange. American cotton (Gossypium hirsutum L.) is the highest fibre producing specie of the world. The present study was conducted to check the comparison of morphological data, fibre quality traits and expression analysis of Cry1Ac gene in 14 cotton genotypes including hybrid (FI), F2 and F4 stages progeny in comparison with check variety MNH-886 at National Agricultural Research Centre, Islamabad during the year 2015 and 2016. The results of morphological traits revealed that field grown cotton genotypes showed variation in morphological characters. The highest plant height was observed 141.6 cm in HSP 7-4(2). Similarly, both maximum number of bolls per plant 115.60 and boll weight 5.92 g was reported in cotton hybrid NIGAB-4 respectively. The best internodes distance 2.32 cm was recorded in B-318-A genotype. There were significant differences observed in morphological traits of cotton except some genotypes. The results of fibre quality traits illustrated that optimum fibre fineness 3.1 µg/inch was reported in cotton hybrid NIGAB-4. While the best results of fibre strength 28.6 g/tex was measured in HSP 7-4(2) and hybrid NIGAB-3, length 33 mm in NIGAB-4, uniformity ratio 49.4 % in NIGAB-4 and fibre elongation 12.9 % was recorded in B1-37 genotype respectively. Meanwhile different Bt genes in 14 cotton genotypes (Cry1Ac, Cry2Ab and Cry1F genes) were checked through immunostrip assay and hence only Mon531 event for Cry1Ac gene of 346 bp was confirmed after immunostrip and PCR analysis. Quantification of Cry1Ac toxin was performed through sandwich ELISA at 80 and 120 days after sowing of leaf and boll tissues of 14 cotton genotypes. The Cry1Ac toxin level of these genotypes is in the range of 0.27 to 3.67µg/g based on fresh weight tissues. From the results, it has been concluded that cotton hybrids have performed well as compared to other tested cotton genotypes.
Key words: American Cotton, Variation, Morphological and fibre quality traits. Cry1Ac toxin. ELISA.
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