SENSITINS FOR DIFFERENTIATING NONSPECIFIC REACTIONS TO PPD TUBERCULIN MAMMALIAN IN CATTLE
M. Bazarbaev1, N. S. Syrym2*, S. Z. Basybekov1, A. K. Mussayeva1, S. G. Kanatbayev3, C. M. Romashev4, A. K. Dossanova5, D. R. Sadikova1, A. T. Mankibaev4 and B. A. Yespembetov2
1Kazakh Scientific Research Veterinary Institute, 223 Raiymbek Avenue, Almaty, 50016, Kazakhstan; 2Scientific Research Institute for Biological Safety Problems, Zhambyl Oblast, GvardeyskiySettlement, 80409, Kazakhstan; 3West Kazakhstan Agrarian Technical University, 54 Zhangir Khan Str., Uralsk, 090009, Kazakhstan; 4Kazakh National Agrarian University, 8 Abaya Avenue, Almaty, 50000, Kazakhstan; 5Kazakh Medical University of Continuing Education, 34 Manasa Str., Almaty, 50040, Kazakhstan
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The paper presents the study of the habitat of mycobacteria in various natural and climatic zones of the Karaganda region, which showed that out of all the isolated cultures, 307 (94.5%) were atypical mycobacteria (М. scrofulaceum, M. avium). At that, 64.5% of atypical mycobacteria cultures (М.scrofulaceum, M. avium) were isolated from the diagnostic material and objects of the external environment of the steppe zone.Thesensitinsfrom epizootic strains of mycobacteria were tested in terms of such criteria as sterility, safety, reactogenicity, lack of sensitizing properties, activity, and specificity. The sensitinswere administered to guinea pigs and cattle using an intradermal needless syringe. Preliminary experiments on guinea pigs found that sensitins had biological and specific activity, identical to avian purified protein derivative tuberculin. The tests of developed sensitinsМ. Scrofulaceumand M. avium on 59 cattle showed that the sensitins were highly effective in differentiating nonspecific reactions and demonstrated the expedience of their application.Using sensitins for lifetime differentiation of nonspecific reactions to tuberculin mammalian in healthy animals and cattle recovering from tuberculosis prevents the premature slaughtering of commercially valuable animals of a reproductive age.
Keywords: atypical mycobacteria, cattle, mycobacteriosis,sensitins, tuberculosis.