POLYMORPHISM OF INHIBIN A GENE AND ITS RELATIONSHIP WITH LITTER SIZE IN CHINESE INDIGENOUS GOAT
Q. Y. Liu1, Y. Q. He2, Y. Ge3, M. X. Chu1*, M. Jin4, Y. J. Zhang5, J. Y. Wang3, X. K. Ma2, R. Di1, D. W. Huang1 and N. Li6
1Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China; 3College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 4College of Life Science, Liaoning Normal University, Dalian 116029, China; 5College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China; 6State Key Laboratory of Agricultural Biotechnology, China Agricultural University, Beijing 100193, China
*Corresponding author’s E-mail address: firstname.lastname@example.org
Inhibin A (INHA) is a glycoprotein hormone which has a role in regulating the synthesis and secretion of pituitary follicle-stimulating hormone. In this paper, single nucleotide polymorphisms of 5′ regulatory region and exon 1 of INHA gene were detected in one prolific goat breed (Jining Grey goat) and five low to medium fecundity breeds (Boer, Liaoning Cashmere, Wendeng Dairy, Taihang and Inner Mongolia Cashmere goats) by PCR-SSCP and sequencing methods. Its effects on litter size in Jining Grey goats were analyzed. Concerning primer P2, two genotypes (AA and Aa) were detected in Jining Grey, Liaoning Cashmere, Wendeng Dairy and Taihang goats. Sequencing revealed a G→A transition at the 258th position of the amplified region in the genotype AA in comparison to the genotype Aa. For primer P4, two genotypes (BB and Bb) were identified in six goat breeds tested. Sequencing revealed a G→A transition at the 759th position of the amplified region in the genotype BB compared with Bb. Jining Grey goat does with genotype Bb had 0.79 (P<0.01) kids more than those with genotype BB. These results preliminarily showed that G841A of the INHA gene was a potential marker for improving litter size in goats.
Key words: goat; INHA gene; prolificacy; PCR-SSCP