EFFECT OF L-CYSTEINE ON POST THAW QUALITY OF NILI RAVI BUFFALO (BUBALUS BUBALIS) BULL SPERMATOZOA CRYOPRESERVED IN TRIS CITRIC ACID EXTENDER
F. Wadood, M. Aleem, A. Ijaz*, N. Ahmad, M. S. Yousaf*, D. H. Mughal*, A. Javid** and ***S. A. Mahmood,
Department of Theriogenology, Department of Physiology*, Department of Wildlife and Ecology**, University of Veterinary and Animal Sciences, Lahore, Pakistan, ***Semen Production Unit, Karani wala, Bahawalpur
Corresponding Author e-mail: profijaz@uvas.edu.pk
ABSTRACT
This study was designed to determine effect of various concentrations of L- cysteine on frozen-thaw semen quality of Nili Ravi buffalo. Single ejaculate of four buffalo bulls (Nili-Ravi) was collected at weekly intervals for 5 weeks. Ejaculates of acceptable motility (≥70%) were pooled and semen was diluted by tris citric acid extender (TCAE; 300 mosm/kg osmotic pressure and ~7.0 pH) having 4 levels of L-cysteine (0.0, 2.0, 5.0 and 8.0 mM). Semen was equilibrated at 4˚C and packaged in 0.5 mL French straws with 20×106 spermatozoa/ straw and then frozen in liquid nitrogen. L-cysteine inclusion levels had non-significant (P> 0.05) effect on spermatozoa motility, viability, NAR, PMI and on lipid peroxidation. However, DNA damage in spermatozoa was significantly higher (P<0.05) in 8.0 mM L-cysteine. It was concluded that L-cysteine (2.0 and 5.0 mM) addition to cryopreserved semen had no significant effect on post thaw semen characteristics, except at 8 mM which adversely affected DNA integrity. This may be due to its toxicity at this concentration.
Key words: cryopreservation, semen, spermatozoa, antioxidants, buffalo, L-cysteine
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