INTER SIMPLE SEQUENCE REPEAT (ISSR) MARKERS AND SOME PHYSIOLOGICAL ATTRIBUTES OF BARLEY (HORDEUM VULGARE L.) GENOTYPES TO DROUGHT AND POTASSIUM NUTRITION
S. A. Akladious and S. M. Abbas*
Biological Science and Geology Department
*Faculty of Education , Ain Shams University, Roxy Cairo, Egypt.
Corresponding Author e-mail: firstname.lastname@example.org
This study was designed to identify useful effects of potassium for drought tolerance in barley. Five genotypes were grown in pot experiment with drought stress levels; 50% and 30% field capacity and potassium sulfate in two levels K1 (40) and K2 (80 mg/ kg). 30% field capacity lead to reduce yield parameters in Giza 130 and Giza 134 genotypes although using of K1 and K2, whereas both K applications increased yield parameters at 50% FC. In Giza 123, 126 and 133, applications of K1 and K2 increased yield parameters under drought stress levels. Based on the obtained results, Giza 126 genotype showed the highest and stable yield across normal and drought conditions. Potassium generally decreased stomata movements of the upper surface of the leaves in the drought stressed plants. SDS-PAGE analysis revealed that plant grown under drought showed induction or suppression in the synthesis of few polypeptides. Giza 126 showed best performance in respect of appearance of new bands in protein profile. ISSR-PCR technique was used to detect some molecular markers associated with drought tolerance. Five ISSR primers were used and revealed 78% polymorphism. The primers produced 12 bands, which could be used as molecular markers in barley breeding programs.
Key words: Drought, Yield, ISSR. Proteins, Anatomical responses.