MICROPROPAGATION OF JARTORPHA CURCAS L. THROUGH SHOOT TIP EXPLANTS USING DIFFERENT CONCENTRATIONS OF PHYTOHORMONES

M. Imtiaz, A. M. Khattak and N. Ara, A. Iqbal* and H. U. Rahman**

Department of Horticulture, Agricultural University Peshawar, Pakistan.
*Institute of Biotechnology and Genetics Engineering, Agricultural University Peshawar, Pakistan.
**Department of Horticulture, Faculty of Agriculture, Gomal University D.I. Khan, Pakistan.
Corresponding Author e-mail: mateen@aup.edu.pk

ABSTRACT

A study was conducted to evaluate the effect of phytohormone BAP alone and in combination with IBA or NAA for in-vitro propagation of Jatropha curcas shoot tip explants. MS medium supplemented with various concentrations of phytohormones significantly affected shoot induction. Maximum (93.33%) shoot induction occurred when MS medium was supplemented with 8.88 µM BAP or 13.32 and 4.92 µM of BAP and IBA respectively. Similarly, maximum shoot buds per explant (6.7) and shoot length (4.5 cm) were obtained when 13.32 µM BAP and 4.92µM IBA was added to the MS medium. Shoots produced were re-cultured to MS, ½ MS media and ½ MS medium supplemented with different concentrations of IBA and were studied for rooting, where the response was highly significant. IBA supplemented to ½ MS medium at 14.76µM gave maximum (4.93) roots per culture.
Key words: Jatropha curcas,micropropagation, phytohormones, BAP, IBA.