DETERMINATION OF SOME BIOCHEMICALS, PHYTOCHEMICALS AND ANTIOXIDANT PROPERTIES OF DIFFERENT PARTS OF Cichorium intybus L.: A COMPARATIVE STUDY
M. A. Shad, H. Nawaz, T. Rehman and N. Ikram
Department of Biochemistry, Department of Chemistry, Bahauddin Zakariya University, Multan-60800, Pakistan
Corresponding Author E-mail: shadaslam@yahoo.com
ABSTRACT
The biochemical, phytochemical and antioxidant composition of root, stem, leaves and seeds of Cichorium intybus L was determined. A statistically significant difference (p>0.05) was observed among different parts of Cichorium intybus regarding the biochemical, phytochemical and antioxidant composition. The leaves were found to possess comparatively higher values of total sugars non-reducing sugars water soluble proteins total flavonoids, total phenolic acids and total antioxidants. On the other hand, seeds were found to possess comparatively higher contents of reducing sugars, salt soluble proteins and saponins. The phytochmical screening confirmed the presence of tannins, saponins, flavonoids, terpenoids, cardiac glycosides and anthocyanins in each part ofthe plant. The antioxidant potential of methanolic extracts of different parts was evaluated in terms of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and reducing power. Theleaf extract was found to show comparatively low value of IC50 for DPPH inhibition and high reducing power. Due to good biochemical, phytochemical and antioxidant composition, Cichorium intybusleaves would be valuable candidate in pharmaceutical formulations and play an important role in improving the human, livestock and poultry health by participating in the antioxidant defence system against endogenous free radicals.
Key words: Antioxidant Composition,Biochemical Analysis, Cichorium intybus, Phytochmical Screening, DPPH radical scavenging capacity. |
