STUDIES ON THE TOXIC EFFECTS OF LEAD AND NICKEL MIXTURE ON TWO FRESHWATER FISHES, CTENOPHARYNGODON IDELLA AND HYPOPHTHALMICHTHYS MOLITRIX
S. Naz and M. Javed
Department of Zoology and Fisheries, University of Agriculture, Faisalabad, Pakistan.
Corresponding author’s e-mail: firstname.lastname@example.org
The Probit Analyses was used to determine the acute toxicity of binary mixture of lead and nickel for two fish species viz. grass carp (Ctenopharyngodon idella) and silver carp (Hypophthalmichthys molitrix). Correlation and regression analyses were also computed to find-out relationships among various parameters. The criteria of toxicity used during these experiments were mortality upon two fish species for 90-day. The tests were performed separately at constant pH (7), temperature (30°C) and hardness (200 mgL-1) of water with three replications for each test dose. There existed significant differences for LC50 and lethal responses for both grass carp and silver carp. The mean LC50 and lethal concentrations for grass carp were56.42±2.51 and 120.98±7.18 while that of silver carp the same were 55.85±2.84and 128.44±9.25 mgL-1,respectively. Among two fish species silver carpwas found significantly more sensitive in terms of 96-hr LC50 than that of grass carp. However, grass carp appeared significantly more sensitive than silver carpfor lethal responses. The metallic ion concentrationsof test mediums showed positively significant correlation with carbon-dioxide, sodium, potassium and electrical conductivity while inverse relationship was identified for dissolved oxygen for both fish species in the test media.
Key words: Acute Toxicity,Metal Mixture, Lead, Nickel,Grass carp, Silver carp.