ANTI-INFLAMMATORY AND ANTI-NOCICEPTIVE ACTIVITIES OF THE EXTRACTS OF SARGENTODOXA CUNEATA AND ITS EFFECTS ON THE MODEL RATS WITH PELVIC INFLAMMATION
Z. H. Zhou, Q. Weng *, J. Zhou ** and J. H. Zhou ***
School of Medicine, Lishui University, Lishui, Zhejiang, China
*The Third People's Hospital of Yuhang District, Hangzhou, Zhejiang, 311115, China
**College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, Zhejiang, 310012, China.
***Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, Zhejiang, China
Corresponding Author’s Email: zjuzhoujh@126.com; juezhou2006@yahoo.com.cn
ABSTRACT
An experiment was conducted to determine the concentration of emodin, the main bioactive content of aqueous extracts of Sargentodoxa cuneata (SC). In addition, the anti-inflammatory, anti-nociceptive activities and the effects of ESC on pelvic inflammation rats were studied. Twenty-one rodents were randomly divided into a SC group, an indomethacin group and a model control group (n=7 in each group) in an ear edema test, a carrageenin-induced paw edema test, a cotton pellet-induced granuloma formation test, and an acetic acid-induced writhing test, respectively. 60 rats were used for pelvic inflammation test. 50 model rats with pelvic inflammation were established and the serum levels of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in each group (n=10) were detected with the Enzyme-Linked ImmunoSorbent Assay (ELISA). Analysis of variance (one-way ANOVA) was employed with 5% significance level (P<0.05) and two-tailed tests were used for all hypothesis tests. The concentration of emodin, a bioactive component in the medicinal plant, was determined by High Performance Liquid Chromatography (HPLC). SC extracts had similar anti-inflammatory and anti-nociceptive activities to indomethacin. ESC significantly decreased the levels of TNF-α and IL-6 of the model rats compared with indomethacin. The content of emodin in ESC was 0.2 mg/g.SC, with its strong anti-inflammatory and anti-nociceptive activities, can be used to treat both acute and chronic inflammation and to relieve the associated pain.
Key words: Sargentodoxa cuneata;anti-inflammatory activities; anti-nociceptive activities; pelvic inflammation; emodin.
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